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eg7 ova  (ATCC)
96
ATCC eg7 ova
(A-C) Detection of ICD markers. Tumor cells were treated with DMSO or ADT-030 for 24 hours. Cell surface expression of CRT was detected by FACS, and MFI values are summarized in (A) as mean ± SEM. Extracellular ATP and HMGB1 levels from the indicated cells were measured and results shown as mean +/- SEM are summarized in (B) and (C), respectively. (D) ADT-030-treated tumor cells promote DC maturation. The schema depicts the experimental setup in which BMDCs were co-cultured with ADT-030 or DMSO pre-treated 4T1.GFP cells for 48 hrs. Uptake of the GFP signal by DCs and DC activation markers (MHC-II, CD80 and CD86) were assessed by FACS. MFIs are summarized as mean ± SEM in bar graphs. (E, F) cDC1-dependent priming of tumor-specific CD8⁺ T cells. The schema depicts the experimental procedures <t>(E).</t> <t>EG7.OVA</t> tumor cells were s.c. implanted to the flanks of WT or Batf3KO mice. Mice with palpable tumors were treated with vehicle or ADT-030. Peripheral blood was collected 10 days after treatment to detect OVA-specific CD8+ T cells by OVA-Kb tetramer. Representative dot plots are shown in (F). Numbers of the gated region demark percent of tetramer-positive cells in CD8+ T cell population. Results shown as mean +/- SEM are summarized in the bar graph. (G) ADT-030 efficacy in mice diminishes in the absence of cDC1. As depicted in the schema, EL4 or KPC cells were s.c. implanted to WT or Batf3KO mice. Mice with palpable tumors were treated daily with vehicle or ADT-030 daily till the endpoint. Tumor growth curves are shown as mean ± SEM, with the number of mice per group indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Eg7 Ova, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e g7 crl 2113 cells  (ATCC)
96
ATCC e g7 crl 2113 cells
(A-C) Detection of ICD markers. Tumor cells were treated with DMSO or ADT-030 for 24 hours. Cell surface expression of CRT was detected by FACS, and MFI values are summarized in (A) as mean ± SEM. Extracellular ATP and HMGB1 levels from the indicated cells were measured and results shown as mean +/- SEM are summarized in (B) and (C), respectively. (D) ADT-030-treated tumor cells promote DC maturation. The schema depicts the experimental setup in which BMDCs were co-cultured with ADT-030 or DMSO pre-treated 4T1.GFP cells for 48 hrs. Uptake of the GFP signal by DCs and DC activation markers (MHC-II, CD80 and CD86) were assessed by FACS. MFIs are summarized as mean ± SEM in bar graphs. (E, F) cDC1-dependent priming of tumor-specific CD8⁺ T cells. The schema depicts the experimental procedures <t>(E).</t> <t>EG7.OVA</t> tumor cells were s.c. implanted to the flanks of WT or Batf3KO mice. Mice with palpable tumors were treated with vehicle or ADT-030. Peripheral blood was collected 10 days after treatment to detect OVA-specific CD8+ T cells by OVA-Kb tetramer. Representative dot plots are shown in (F). Numbers of the gated region demark percent of tetramer-positive cells in CD8+ T cell population. Results shown as mean +/- SEM are summarized in the bar graph. (G) ADT-030 efficacy in mice diminishes in the absence of cDC1. As depicted in the schema, EL4 or KPC cells were s.c. implanted to WT or Batf3KO mice. Mice with palpable tumors were treated daily with vehicle or ADT-030 daily till the endpoint. Tumor growth curves are shown as mean ± SEM, with the number of mice per group indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
E G7 Crl 2113 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e g7 crl 2113 cells/product/ATCC
Average 96 stars, based on 1 article reviews
e g7 crl 2113 cells - by Bioz Stars, 2026-03
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mouse e g7 lymphoblast cell line expressing ova  (ATCC)
97
ATCC mouse e g7 lymphoblast cell line expressing ova
(A-C) Detection of ICD markers. Tumor cells were treated with DMSO or ADT-030 for 24 hours. Cell surface expression of CRT was detected by FACS, and MFI values are summarized in (A) as mean ± SEM. Extracellular ATP and HMGB1 levels from the indicated cells were measured and results shown as mean +/- SEM are summarized in (B) and (C), respectively. (D) ADT-030-treated tumor cells promote DC maturation. The schema depicts the experimental setup in which BMDCs were co-cultured with ADT-030 or DMSO pre-treated 4T1.GFP cells for 48 hrs. Uptake of the GFP signal by DCs and DC activation markers (MHC-II, CD80 and CD86) were assessed by FACS. MFIs are summarized as mean ± SEM in bar graphs. (E, F) cDC1-dependent priming of tumor-specific CD8⁺ T cells. The schema depicts the experimental procedures <t>(E).</t> <t>EG7.OVA</t> tumor cells were s.c. implanted to the flanks of WT or Batf3KO mice. Mice with palpable tumors were treated with vehicle or ADT-030. Peripheral blood was collected 10 days after treatment to detect OVA-specific CD8+ T cells by OVA-Kb tetramer. Representative dot plots are shown in (F). Numbers of the gated region demark percent of tetramer-positive cells in CD8+ T cell population. Results shown as mean +/- SEM are summarized in the bar graph. (G) ADT-030 efficacy in mice diminishes in the absence of cDC1. As depicted in the schema, EL4 or KPC cells were s.c. implanted to WT or Batf3KO mice. Mice with palpable tumors were treated daily with vehicle or ADT-030 daily till the endpoint. Tumor growth curves are shown as mean ± SEM, with the number of mice per group indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Mouse E G7 Lymphoblast Cell Line Expressing Ova, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse e g7 lymphoblast cell line expressing ova/product/ATCC
Average 97 stars, based on 1 article reviews
mouse e g7 lymphoblast cell line expressing ova - by Bioz Stars, 2026-03
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biological sex  (ATCC)
96
ATCC biological sex
(A-C) Detection of ICD markers. Tumor cells were treated with DMSO or ADT-030 for 24 hours. Cell surface expression of CRT was detected by FACS, and MFI values are summarized in (A) as mean ± SEM. Extracellular ATP and HMGB1 levels from the indicated cells were measured and results shown as mean +/- SEM are summarized in (B) and (C), respectively. (D) ADT-030-treated tumor cells promote DC maturation. The schema depicts the experimental setup in which BMDCs were co-cultured with ADT-030 or DMSO pre-treated 4T1.GFP cells for 48 hrs. Uptake of the GFP signal by DCs and DC activation markers (MHC-II, CD80 and CD86) were assessed by FACS. MFIs are summarized as mean ± SEM in bar graphs. (E, F) cDC1-dependent priming of tumor-specific CD8⁺ T cells. The schema depicts the experimental procedures <t>(E).</t> <t>EG7.OVA</t> tumor cells were s.c. implanted to the flanks of WT or Batf3KO mice. Mice with palpable tumors were treated with vehicle or ADT-030. Peripheral blood was collected 10 days after treatment to detect OVA-specific CD8+ T cells by OVA-Kb tetramer. Representative dot plots are shown in (F). Numbers of the gated region demark percent of tetramer-positive cells in CD8+ T cell population. Results shown as mean +/- SEM are summarized in the bar graph. (G) ADT-030 efficacy in mice diminishes in the absence of cDC1. As depicted in the schema, EL4 or KPC cells were s.c. implanted to WT or Batf3KO mice. Mice with palpable tumors were treated daily with vehicle or ADT-030 daily till the endpoint. Tumor growth curves are shown as mean ± SEM, with the number of mice per group indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Biological Sex, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biological sex/product/ATCC
Average 96 stars, based on 1 article reviews
biological sex - by Bioz Stars, 2026-03
96/100 stars
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e g7 ova  (ATCC)
96
ATCC e g7 ova
(A-C) Detection of ICD markers. Tumor cells were treated with DMSO or ADT-030 for 24 hours. Cell surface expression of CRT was detected by FACS, and MFI values are summarized in (A) as mean ± SEM. Extracellular ATP and HMGB1 levels from the indicated cells were measured and results shown as mean +/- SEM are summarized in (B) and (C), respectively. (D) ADT-030-treated tumor cells promote DC maturation. The schema depicts the experimental setup in which BMDCs were co-cultured with ADT-030 or DMSO pre-treated 4T1.GFP cells for 48 hrs. Uptake of the GFP signal by DCs and DC activation markers (MHC-II, CD80 and CD86) were assessed by FACS. MFIs are summarized as mean ± SEM in bar graphs. (E, F) cDC1-dependent priming of tumor-specific CD8⁺ T cells. The schema depicts the experimental procedures <t>(E).</t> <t>EG7.OVA</t> tumor cells were s.c. implanted to the flanks of WT or Batf3KO mice. Mice with palpable tumors were treated with vehicle or ADT-030. Peripheral blood was collected 10 days after treatment to detect OVA-specific CD8+ T cells by OVA-Kb tetramer. Representative dot plots are shown in (F). Numbers of the gated region demark percent of tetramer-positive cells in CD8+ T cell population. Results shown as mean +/- SEM are summarized in the bar graph. (G) ADT-030 efficacy in mice diminishes in the absence of cDC1. As depicted in the schema, EL4 or KPC cells were s.c. implanted to WT or Batf3KO mice. Mice with palpable tumors were treated daily with vehicle or ADT-030 daily till the endpoint. Tumor growth curves are shown as mean ± SEM, with the number of mice per group indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
E G7 Ova, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e g7 ova/product/ATCC
Average 96 stars, based on 1 article reviews
e g7 ova - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
e g7 ova cells  (ATCC)
96
ATCC e g7 ova cells
(A-C) Detection of ICD markers. Tumor cells were treated with DMSO or ADT-030 for 24 hours. Cell surface expression of CRT was detected by FACS, and MFI values are summarized in (A) as mean ± SEM. Extracellular ATP and HMGB1 levels from the indicated cells were measured and results shown as mean +/- SEM are summarized in (B) and (C), respectively. (D) ADT-030-treated tumor cells promote DC maturation. The schema depicts the experimental setup in which BMDCs were co-cultured with ADT-030 or DMSO pre-treated 4T1.GFP cells for 48 hrs. Uptake of the GFP signal by DCs and DC activation markers (MHC-II, CD80 and CD86) were assessed by FACS. MFIs are summarized as mean ± SEM in bar graphs. (E, F) cDC1-dependent priming of tumor-specific CD8⁺ T cells. The schema depicts the experimental procedures <t>(E).</t> <t>EG7.OVA</t> tumor cells were s.c. implanted to the flanks of WT or Batf3KO mice. Mice with palpable tumors were treated with vehicle or ADT-030. Peripheral blood was collected 10 days after treatment to detect OVA-specific CD8+ T cells by OVA-Kb tetramer. Representative dot plots are shown in (F). Numbers of the gated region demark percent of tetramer-positive cells in CD8+ T cell population. Results shown as mean +/- SEM are summarized in the bar graph. (G) ADT-030 efficacy in mice diminishes in the absence of cDC1. As depicted in the schema, EL4 or KPC cells were s.c. implanted to WT or Batf3KO mice. Mice with palpable tumors were treated daily with vehicle or ADT-030 daily till the endpoint. Tumor growth curves are shown as mean ± SEM, with the number of mice per group indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
E G7 Ova Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e g7 ova cells/product/ATCC
Average 96 stars, based on 1 article reviews
e g7 ova cells - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier

Image Search Results


(A-C) Detection of ICD markers. Tumor cells were treated with DMSO or ADT-030 for 24 hours. Cell surface expression of CRT was detected by FACS, and MFI values are summarized in (A) as mean ± SEM. Extracellular ATP and HMGB1 levels from the indicated cells were measured and results shown as mean +/- SEM are summarized in (B) and (C), respectively. (D) ADT-030-treated tumor cells promote DC maturation. The schema depicts the experimental setup in which BMDCs were co-cultured with ADT-030 or DMSO pre-treated 4T1.GFP cells for 48 hrs. Uptake of the GFP signal by DCs and DC activation markers (MHC-II, CD80 and CD86) were assessed by FACS. MFIs are summarized as mean ± SEM in bar graphs. (E, F) cDC1-dependent priming of tumor-specific CD8⁺ T cells. The schema depicts the experimental procedures (E). EG7.OVA tumor cells were s.c. implanted to the flanks of WT or Batf3KO mice. Mice with palpable tumors were treated with vehicle or ADT-030. Peripheral blood was collected 10 days after treatment to detect OVA-specific CD8+ T cells by OVA-Kb tetramer. Representative dot plots are shown in (F). Numbers of the gated region demark percent of tetramer-positive cells in CD8+ T cell population. Results shown as mean +/- SEM are summarized in the bar graph. (G) ADT-030 efficacy in mice diminishes in the absence of cDC1. As depicted in the schema, EL4 or KPC cells were s.c. implanted to WT or Batf3KO mice. Mice with palpable tumors were treated daily with vehicle or ADT-030 daily till the endpoint. Tumor growth curves are shown as mean ± SEM, with the number of mice per group indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Journal: bioRxiv

Article Title: Targeting phosphodiesterase 10A disrupts MAPK signaling pathways in the tumor microenvironment to unleash antitumor immunity

doi: 10.64898/2026.02.26.708383

Figure Lengend Snippet: (A-C) Detection of ICD markers. Tumor cells were treated with DMSO or ADT-030 for 24 hours. Cell surface expression of CRT was detected by FACS, and MFI values are summarized in (A) as mean ± SEM. Extracellular ATP and HMGB1 levels from the indicated cells were measured and results shown as mean +/- SEM are summarized in (B) and (C), respectively. (D) ADT-030-treated tumor cells promote DC maturation. The schema depicts the experimental setup in which BMDCs were co-cultured with ADT-030 or DMSO pre-treated 4T1.GFP cells for 48 hrs. Uptake of the GFP signal by DCs and DC activation markers (MHC-II, CD80 and CD86) were assessed by FACS. MFIs are summarized as mean ± SEM in bar graphs. (E, F) cDC1-dependent priming of tumor-specific CD8⁺ T cells. The schema depicts the experimental procedures (E). EG7.OVA tumor cells were s.c. implanted to the flanks of WT or Batf3KO mice. Mice with palpable tumors were treated with vehicle or ADT-030. Peripheral blood was collected 10 days after treatment to detect OVA-specific CD8+ T cells by OVA-Kb tetramer. Representative dot plots are shown in (F). Numbers of the gated region demark percent of tetramer-positive cells in CD8+ T cell population. Results shown as mean +/- SEM are summarized in the bar graph. (G) ADT-030 efficacy in mice diminishes in the absence of cDC1. As depicted in the schema, EL4 or KPC cells were s.c. implanted to WT or Batf3KO mice. Mice with palpable tumors were treated daily with vehicle or ADT-030 daily till the endpoint. Tumor growth curves are shown as mean ± SEM, with the number of mice per group indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Article Snippet: Murine tumor cell lines 4T1, A20, B16F10, CT26, EL4, EG7.OVA and MC38 were purchased from ATCC.

Techniques: Expressing, Cell Culture, Activation Assay

(A) ADT-030 exhibits curative antitumor effect in EG7.OVA tumor model. Following the workflow depicted in the schema, WT C57BL/6 mice were implanted s.c. with EG7.OVA tumor cells. Mice with established tumors (50-100mm2) were treated with ADT-030 for 12 consecutive days. 3 mice that achieved complete tumor regression were rechallenged with EG7.OVA tumor cells. Naïve mice inoculated with EG7.OVA tumors at the same time servied as controls. (B) ADT-030 antitumor efficacy is compromised in Batf3KO mice. Following the workflow depicted in the schema, EG7.OVA cells were s.c. implanted to WT or Batf3KO mice. Mice with established tumors were treated daily with vehicle or ADT-030 daily till the endpoint. Tumor growth curves are shown as mean ± SEM, with the number of mice per group indicated. *, P < 0.05.

Journal: bioRxiv

Article Title: Targeting phosphodiesterase 10A disrupts MAPK signaling pathways in the tumor microenvironment to unleash antitumor immunity

doi: 10.64898/2026.02.26.708383

Figure Lengend Snippet: (A) ADT-030 exhibits curative antitumor effect in EG7.OVA tumor model. Following the workflow depicted in the schema, WT C57BL/6 mice were implanted s.c. with EG7.OVA tumor cells. Mice with established tumors (50-100mm2) were treated with ADT-030 for 12 consecutive days. 3 mice that achieved complete tumor regression were rechallenged with EG7.OVA tumor cells. Naïve mice inoculated with EG7.OVA tumors at the same time servied as controls. (B) ADT-030 antitumor efficacy is compromised in Batf3KO mice. Following the workflow depicted in the schema, EG7.OVA cells were s.c. implanted to WT or Batf3KO mice. Mice with established tumors were treated daily with vehicle or ADT-030 daily till the endpoint. Tumor growth curves are shown as mean ± SEM, with the number of mice per group indicated. *, P < 0.05.

Article Snippet: Murine tumor cell lines 4T1, A20, B16F10, CT26, EL4, EG7.OVA and MC38 were purchased from ATCC.

Techniques: